Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis
نویسندگان
چکیده
منابع مشابه
Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene.
The Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, Calif.) utilizes capillary electrophoresis on a microchip device (LabChip 7500; Caliper Technologies, Mountain View, Calif.) that is capable of rapidly sizing small DNA fragments. To determine whether the system could replace conventional restriction fragment length polymorphism (RFLP) typing by agarose gel electrophoresis, we compa...
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Four flagellin allelic types (I to IV) of Burkholderia pseudomallei were identified based on their sequence variation and restriction fragment length polymorphism (RFLP) analysis of the amplified flagellin gene. Flagellin allelic type I was the most predominantly (75.0%) found among the 100 clinical isolates of B. pseudomallei investigated in this study.
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Amplified-fragment length polymorphism (AFLP) analysis with the endonucleases BglII and MfeI was used to genotype 91 Campylobacter jejuni subsp. jejuni strains from outbreaks and sporadic cases. AFLP-generated fragments were labeled with fluorescent dye and separated by capillary electrophoresis. The software packages GeneScan and GelCompar II were used to calculate AFLP pattern similarities an...
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Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis is a recently developed technique which allows strains of tuberculosis to be differentiated with a high degree of confidence. The application of this technique has enabled the patterns of transmission of tuberculosis to be elucidated with far greater certainty than was previously possible. This review will disc...
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Twenty one staphylococcus aureus isolates of bovine mastitic milk origin from herds at different locations were analysed for their coa gene products and RFLP patterns. One of the isolates was coa gene deficient whereas the rest revealed polymorphism in the coa gene. The isolates revealed three different types of coa gene products (600, 680 or 850 bp) and three distinct RFLP patterns were obtain...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.6.1531-1536.1993